Smartphone Instrumentations for Public Health Safety by Abbas Jamalipour & Md Arafat Hossain

Author:Abbas Jamalipour & Md Arafat Hossain
Language: eng
Format: epub
ISBN: 9783030020958
Publisher: Springer International Publishing

Figure 3.2b shows the interior design of the sample chamber. The main components in this chamber are slots for a sample cell, the excitation source and the emission filter. The sample slot is designed considering a quartz cuvette cell of path length, l = 1 cm. The excitation source is 3 mm single UV LED mounted onto a 2 × 2 cm mini PCB attached to the side-wall of the sample chamber. The excitation filter slot is designed to fit a thin film filter of maximum dimension, 5 cm × 2.5 cm × 1 mm. The cuvette cell is designed in such a way that one side of the cell is enclosed by the surface of a Peltier, making efficient contact between the sample cell and Peltier’s hot/cold surface. This allows efficient heat transfer between the Peltier and sample. In order to reduce the levels of UV radiation reaching the CMOS camera directly and maximize the fluorescence readout, the UV LED is positioned orthogonal to the fluorescence emission path from the sample contained in a (1 × 1 × 4.5) cm quartz cuvette. An emission filter at λem ~ 450 nm is used to further reduce background scattering and allowed blue fluorescence to pass which can be replaced or complemented by other bandpass filters where required. The main fluorimeter box contains a window at the top of the sample cuvette chamber so that the user can easily access the sample in the chamber; this can be closed by a 3D-printed cover once the sample is in place. Temperature of the sample was detected using an IR T-sensor. A round slot in the fluorimeter box, just underneath of the sample cuvette unit, holds the T-sensor. The complete smartphone fluorimeter design after placing all components in position is shown in Fig. 3.2d. Once the design is completed, the individual part of the fluorimeter is fabricated with a 3D printer using ABS material of black color and assembled to build the complete smartphone intensity fluorimeter as shown in Fig. 3.3.

Fig. 3.33D-printed Arduino-based temperature tunable smartphone fluorimeter installed on a Blackview ZETA smartphone [125]

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